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Maxells™
3D expansion of stem cells
Traditional pluripotent stem cells are grown as adherent cultures, with limited and expensive scalability.Accellta solves the scale-up and cost challenges of stem cell culturing using the Maxells™ and Singles solutions.
Maxells™ are stem cells cultured as cell clusters devoid of feeder cells without micro-carriers. Using dynamic bioreactors, Maxells™ provides a vastly cost-effective means to expand stem cells in customized quantities.
Maxells™ delivers unprecedented magnitude and quality. Based on proprietary protocols developed by Accellta, Maxells technology can be applied to create both research-grade as well as clinical-grade feeder-free xeno-free cultures. In addition, Accellta developed proprietary protocols for the differentiation of the Maxells directly in suspension to a variety of cell types (e. g., MSCs. HSCs, NPs, CMs)
- Cell concentration: >20 million cells/ml
- Expansion factor: X30-35 in 11 days
- Suitable for undifferentiated pluripotent stem cells and progenitor cells
- Does not require enzymatic splitting
- Rock inhibitor-free, xeno-free defined medium
- Medium easily refreshed every two-three days
- Straightforward perfusion process in bioreactors
- Easy transition from suspension culture back to adherent culture
- Feeder cell-free
- Homogenous exposure to medium’s nutrients
- >95% of cells are pluripotent; <5% spontaneous differentiation
- Karyotype stability
- High viability rate
- Methods for directed differentiation in suspension