Accellta provides tailor made expansion, differentiation and bio-manufacturing solutions using its proprietary 3D suspension technologies and know-how. The services include process development, cell banking and comprehensive media services ranging from media formulation, media development to screening of media components and media optimization), The company’s process development solutions include:

1. Process development services for culturing stem cells and or their derivatives in feeder-free, xeno-free, serum-free 3D non-adherent conditions in spinners and bioreactors.
2. Process development services for differentiation of stem cells to the desired cell type in feeder-free, xeno-free, serum-free 3D non-adherent conditions in spinners or bioreactors.
3. Protocols for culturing and differentiation of pluripotent stem cells in feeder free 2D adherent conditions.

Accellta developed proprietary culture systems and formulations for animal product-free and serum-free culturing of pluripotent stem cells. The systems include media that are supplemented with recombinant human growth factors and are suitable for growing hPSCs. Some of the offered medium formulations are suitable for GMP manufacturing.
Accellta has proprietary xeno-free media formulations for both suspension cultures and adherent cultures on either extracellular matrices, such as fibronectin, laminin and Matrigel ^TM, as well as human foreskin-derived fibroblast feeder cells. In addition, we offer culture medium formulations for directed differentiation of PSCs in suspension culture.

Screening for GF optimal concentration and/or serum replacements performance on 2D or 3D suspension culture

1. Establishment of a uniform fully characterized master cell bank (MCB) and working cell bank (WCB).

2. Option for GMP-compliant cell bank